Main sol. 328
Internal Solution-ID: S602
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 5.0 | g | 5 | - | |
|
Trypticase
(BBL) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| Mineral solution | 40.0 | ml | - | - | |
| (NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
| Fatty acid mixture | 3.1 | ml | - | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
|
Haemin
(dissolved in 0.5 ml 1 N NaOH) |
5.0 | mg | 0.005 | 0.008 | |
|
Vitamin K1
(0.5% sol. in ethanol) |
0.2 | ml | 1.0e-3 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Adjust pH to 6.9. | |||||
| 2 Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. | |||||
Media with this solution
| 328 | PYG MEDIUM WITH VOLATILE FATTY ACIDS |