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Mail sol. C41

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Internal Solution-ID: S6171

This solution is used in 2 media and was first defined in medium C41.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		0.7 ml - -
KH2PO4
(2.48 g/200 ml stock solution) 		0.7 ml 1.638 12.037
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		0.7 ml - -
NaHCO3
(3.18 g/200 ml stock solution) 		0.7 ml - -
Solution 1 0.7 ml - -
Solution 2 0.7 ml - -
Solution 3 0.7 ml - -
NaNO3
(16.0 g/200 ml stock solution) 		0.7 ml - -
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		0.7 ml - -
SE2 (Soil Extract 2) 300.0 ml - -
Deionized water 1000.0 ml - -
Agar , for solid medium 15.0 g 15 -
1 This recipe is a 7:3 mixture of JM and SE2. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
C41 JM:SE
C89 SBBM