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CCAP 41 . JM:SE

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C41 #

ml
Solution occurs in 1 other media (first defined in Medium C41).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		0.7 ml - -
KH2PO4
(2.48 g/200 ml stock solution) 		0.7 ml 1.638 12.037
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		0.7 ml - -
NaHCO3
(3.18 g/200 ml stock solution) 		0.7 ml - -
Solution 1 0.7 ml - -
Solution 2 0.7 ml - -
Solution 3 0.7 ml - -
NaNO3
(16.0 g/200 ml stock solution) 		0.7 ml - -
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		0.7 ml - -
SE2 (Soil Extract 2) 300.0 ml - -
Deionized water 1000.0 ml - -
Agar , for solid medium 15.0 g 15 -
1 This recipe is a 7:3 mixture of JM and SE2. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.

Solution 1 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 0.45 g 2.25 -
Na2-EDTA 0.45 g 2.25 6.692

Solution 2 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 0.496 g 2.48 40.104
MnCl2 x 4 H2O 0.278 g 1.39 7.024
(NH4)6Mo7O24 x 4 H2O 0.200 g 1 0.809

Solution 3 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 0.008 g 0.04 0.03
Thiamine HCl 0.008 g 0.04 0.119
Biotine 0.008 g 0.04 0.164

SE2 (Soil Extract 2) #

1000
Solution occurs in 13 other media (first defined in Medium C12).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried) 		trace - -
Distilled water trace - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3 Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 34
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 -
KH2PO4 1.638 12.037
H3BO3 0.002 0.028
Na2-EDTA 0.002 0.005
FeNa-EDTA 0.002 -
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