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Trace elements solution

Internal Solution-ID: S621

This solution is used in 2 media and was first defined in medium 334.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl3 x 6 H2O 1.35 g 1.35 4.995
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.03 g 0.03 0.231
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.03 g 0.03 0.223
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
NiCl2 x 6 H2O 0.12 g 0.12 0.926
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
334a METHANOTHRIX SOEHNGENII MEDIUM
334 METHANOTHRIX MEDIUM