Na-syringate solution (0.3 M)
Internal Solution-ID: S6318
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Syringic acid
(SIGMA S6881) |
60 | g | 60 | - | |
NaOH
(5 N) |
50 | ml | - | - | |
Distilled water | 950 | ml | - | - | |
1 Add syringic acid to 700 ml distilled water, add about 50 ml of 5 N NaOH and adjust the pH to about 7.5 to dissolve it. Then make up to a volume of 1000 ml with water. Make the solution anoxic by sparging with 100% N2 gas, distribute in anoxic serum vials and autoclave at 121°C. |
135b | ACETOBACTERIUM DEHALOGENANS MEDIUM |