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CYE-ACES alpha AGAR

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Internal Solution-ID: S6330

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 490 ml - -
Solution B 490 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Prepare OXOID Legionella CYE-Agar base or solution A.
2 Cool down to 50 °C.
3 Add solution C (Cysteine-HCl) and solution D (ferric pyrophosphate).
4 Adjust pH to 6.9 with sterile 1.0 N KOH or HCl.
5 The pH of the medium is critical.
6 Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.
585a BCYE alpha agar