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DSMZ

DSMZ 585a . BCYE alpha agar

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

CYE-ACES alpha AGAR #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 490 ml - -
Solution B 490 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Prepare OXOID Legionella CYE-Agar base or solution A.
2 Cool down to 50 °C.
3 Add solution C (Cysteine-HCl) and solution D (ferric pyrophosphate).
4 Adjust pH to 6.9 with sterile 1.0 N KOH or HCl.
5 The pH of the medium is critical.
6 Finally, add solution B. Swirl medium in flask during dispensing to petri dishes or tubes in order to keep charcoal suspended.

OXOID Legionella CYE-Agar base #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
OXOID Legionella CYE-Agar base
(with Legionella BCYE supplement) 		1000 ml - -
1 Dehydrated media preparations are available from media distributors.
2 We use OXOID Legionella CYE-Agar base with Legionella BCYE supplement.
3 Note: Alternatively use CYE-ACES AGAR

Solution A #

490
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
ACES
(N-2-acetamido-2-aminoethane-sulfonic acid) 		10 g 20.408 112.01
Yeast extract
(Difco 0127) 		10 g 20.408 -
Activated charcoal
(Sigma C 5510) 		2 g 4.082 -
Na2 alpha-ketoglutarate 1 g 2.041 14.164
Distilled water 490 ml - -
1 Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C.

Solution B #

490
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Agar 15 g 30.612 -
Distilled water 490 ml - -
1 Autoclave at 121°C and cool to 50 - 55°C.

Solution C #

10
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Cysteine HCl x H2O 0.4 g 40 227.744
Distilled water 10.0 ml - -
1 Prepare a fresh solution and filter-sterilize it.

Solution D #

10
Solution occurs in 1 other media (first defined in Medium 585).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe4(PO4)2 0.25 g 25 33.548
Distilled water 10.00 ml - -
1 Prepare a fresh solution by heating to 50 - 55°C in a water-bath. Filter-sterilize solution separately.
2 Please note: Soluble ferric pyrophosphate must be kept dry and in the dark. Do not use if chemical looses its green colour and becomes brown or yellow.

Metadata

Last modified: 15.05.23
Source: MediaDive: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 -
ACES 10 54.885
Yeast extract 10 -
Activated charcoal 2 -
Na2 alpha-ketoglutarate 1 6.941
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Cultivation metadata from 2 strains BacDive

pH 4 - 6 2 strains
pH 6 - 8 2 strains
phyla 1 2 strains
oxygen aerobe 2 strains
oxygen obligate aerobe 2 strains
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