Modified Vitamin Elixir
Internal Solution-ID: S6341
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Thiamin | 90.0 | mg | 0.9 | 3.392 | |
| Inositol | 40.0 | mg | 0.4 | 2.22 | |
|
DL-Calcium pantothenate
(2 mg/ml) |
4.0 | ml | - | - | |
|
p-Aminobenzoic acid
(2 mg/ml) |
2.5 | ml | - | - | |
|
Vitamin B12
(1 mg/ml) |
5.0 | ml | - | - | |
|
Pyridoxine
(1 mg/ml) |
4.0 | ml | - | - | |
|
Nicotinic acid
(1 mg/ml) |
4.0 | ml | - | - | |
|
Biotin
, needs to be warmed (50°C)
(0.1 mg/ml) |
1.0 | ml | - | - | |
|
Folic acid
, suspension
(0.1 mg/ml) |
0.4 | ml | - | - | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Add thiamine and inositol to approx. 50 mL distilled deionized water. | |||||
| 2 Then add stock vitamins, and bring up volume to 100 mL in a volumetric flask. | |||||
| 3 Filter sterilize (0.2 µm) and store at 4°C | |||||
| 4 Note: * Biotin stock will need to be warmed (to about 50oC) to get it into solution. ** Folic acid stock is more like a suspension than a solution; make sure it is well-mixed before taking aliquot out. It will dissolve in final solution. | |||||