Main sol. 88d
Internal Solution-ID: S6365
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.287 | 9.741 | |
| KH2PO4 | 0.28 | g | 0.277 | 2.037 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.248 | 1.004 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.069 | 0.471 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.073 | |
| Distilled water | 1000.00 | ml | - | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| 1 Adjust pH to 1.0 with 1N H2SO4. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 After autoclaving add the following sterile solutions: | |||||
|
Betaine Hydrochloride
(10% solution in water, filter-sterilized) |
6.00 | ml | 0.594 | - | |
|
Kao and Michayluk vitamin solution
(HM-PL020) |
10.00 | ml | - | - | |
|
Beef extract
, (BD)
(BD-Difco, 10% solution in water, autoklaved) |
10.00 | ml | 0.99 | - | |
|
Bacto Tryptone
(BD-Difco, 10% solution in water, autoklaved) |
10.00 | ml | 0.99 | - | |