Main sol. 545c
Internal Solution-ID: S6398
Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.3 | g | 0.3 | 1.708 | |
| Na-thioglycolate | 0.3 | g | 0.3 | 2.629 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine and thioglycolate), boil medium for 1 minute, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and thioglycolate, then adjust the pH to 8.0. Dispense medium under the same gas atmosphere in culture vessels and autoclave at 121ºC for 15 minutes. Check and adjust the pH of the complete medium to 8.0, if necessary. | |||||
Media with this solution
| 545c | TRYPTONE SOYA BROTH (ANAEROB) |