Main sol. 1183a
Internal Solution-ID: S6449
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.3 | g | 0.3 | 4.024 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| MgSO4 | 2.0 | g | 2 | 16.615 | |
| NaCl | 15.0 | g | 15 | 256.674 | |
| Sodium succinate | 1.0 | g | 1 | 6.171 | |
| Na acetate | 1.0 | g | 1 | 12.19 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Bacto peptone | 0.5 | g | 0.5 | - | |
| Trace elements solution | 2.0 | ml | - | - | |
| Vitamin solution | 2.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH is about 5.9 before autoclaving. | |||||
| 2 Add to the autoclaved medium the following, from sterile stock solutions | |||||
|
K2HPO4
(10%) |
3.0 | ml | 0.3 | 1.722 | |
|
NaHCO3
(10%) |
5.0 | ml | 0.5 | 5.952 | |
|
CaCl2
(10%) |
0.5 | ml | 0.05 | 0.451 | |
| 3 The pH of the final medium should be 8.0-8.5. | |||||
Media with this solution
| 1183a | Salinarimonas Medium |