Main sol. 348
Internal Solution-ID: S687
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 120.00 | g | 120 | 2053.39 | |
| MgCl2 x 6 H2O | 7.00 | g | 7 | 34.431 | |
| MgSO4 x 7 H2O | 6.00 | g | 6 | 24.343 | |
| CaCl2 x 2 H2O | 0.50 | g | 0.5 | 3.401 | |
| K2HPO4 x 3 H2O | 0.40 | g | 0.4 | 2.297 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KCl | 3.80 | g | 3.8 | 50.973 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
| Trimethylamine-HCl | 1.90 | g | 1.9 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add cysteine and adjust pH to 6.5, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 7.3 - 7.4, if necessary. | |||||
Media with this solution
| 348 | METHANOHALOPHILUS PORTUCALENSIS MEDIUM |