MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 354

Download this solution as

CSV JSON PDF

Internal Solution-ID: S694

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.50 g 1.551 11.396
MgSO4 0.24 g 0.149 1.237
Mg-citrate 0.60 g 0.372 1.736
L-Asparagin 3.60 g 2.233 16.903
Potato flour 30.00 g 18.61 -
Malachite green 0.40 g 0.248 0.68
Distilled water 600.00 ml - -
1 Addition:
Glycerol 12.00 ml - -
Fresh egg mixture
(yolk and whites, *) 		1000.00 ml - -
2 pH 7.0
3 Sterilize medium for 30 min at 121°C, cool to 30°C and add fresh egg mixture carefully to the medium.
4 * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully.
354 LÖWENSTEIN-JENSEN MEDIUM