Main sol. 382
Internal Solution-ID: S727
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
10 x M9 salts (per l) | 100 | ml | - | - | |
MgSO4
(1 M) |
1 | ml | 0.12 | 1 | |
CaCl2
(0.1 M) |
1 | ml | 0.011 | 0.1 | |
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M) |
1 | ml | 0.373 | 1 | |
Glucose
(20%) |
10 | ml | 2 | 11.101 | |
Distilled water | 900 | ml | - | - | |
1 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. | |||||
2 Adjust pH to 7.4. |
382 | MINERAL MEDIUM M9 for E. coli JM strains |