Main sol. 414
Internal Solution-ID: S849
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 5 | - | |
| Casamino acids | 10.0 | g | 10 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| Glucose | 5.0 | g | 5 | 27.753 | |
| Na glutamate | 4.0 | g | 4 | 23.653 | |
| Arginine | 1.0 | g | 1 | 5.741 | |
| Glycine | 1.0 | g | 1 | 13.321 | |
| DL-Tryptophan | 0.1 | g | 0.1 | 0.49 | |
| KH2PO4 | 2.0 | g | 2 | 14.697 | |
| Tween 80 | 0.5 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 pH 7.0 - 7.2. | |||||
| 2 Prepare and distribute medium anaerobically using the Hungate technique | |||||
Media with this solution
| 414 | ACIDAMINOCOCCUS FERMENTANS MEDIUM |