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Main sol. 429b

Internal Solution-ID: S870

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Horse blood OR Sheep blood 10 % 100 -
1 Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80°C for 10 min. Mix well and pour plates after cooling to 50°C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1.