429b
429b .
CHOCOLATE AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 429b #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia agar base | 1000 | ml | - | - | |
| Horse blood OR Sheep blood | 10 | % | 100 | - | |
| 1 Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80°C for 10 min. Mix well and pour plates after cooling to 50°C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , for solid medium | 15 | g | 15 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Solution occurs in 22
other media (first defined in Medium 220).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 15 | g | - | - | |
| Soy peptone | 5 | g | - | - | |
| NaCl | 5 | g | - | - | |
| Agar | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||
Solution occurs in 5
other media (first defined in Medium 215).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 19.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Horse blood | 100 | - |
| Sheep blood | 100 | - |
| Columbia agar base | - | - |
