DSMZ 429 . COLUMBIA BLOOD AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000 ml - -
Horse blood 4 % 40 -
1 Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion).
Solution occurs in 4 other media (first defined in Medium 54a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 17.0 g 17 -
Soy peptone 3.0 g 3 -
D(+)-Glucose 2.5 g 2.5 13.877
NaCl 5.0 g 5 85.558
K2HPO4 2.5 g 2.5 14.353
Distilled water 1000.0 ml - -
1 Adjust pH to 7.3.
2 The same as Caso Bouillon (Merck or Oxoid, Germany).
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -
Solution occurs in 2 other media (first defined in Medium 429).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 5 g 5 -
Meat extract 3 g 3 -
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Metadata

Last modified: 20.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 109
Strains with modifications: 42

Media similar to medium 429

438 BORDET-GENGOU-MEDIUM (DIFCO)
429e e. COLUMBIA BLOOD AGAR 10%
420 BLOOD AGAR I

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 40 -
Columbia agar base - -

Cultivation metadata from 109 strains BacDive

halophily halotolerant 1 strain
pH 6 - 8 3 strains
phyla 4 110 strains
nutrition chemoorganotroph 2 strains
oxygen microaerophile 42 strains
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