DSMZ 429c . COLUMBIA-GLUCOSE-CYSTEINE-AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000.0 ml - -
Horse blood OR Sheep blood 4.0 % 40 -
Glucose 1.0 % 10 55.506
Cysteine 0.1 % 1 8.254
1 Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.
Solution occurs in 22 other media (first defined in Medium 220).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 15 g - -
Soy peptone 5 g - -
NaCl 5 g - -
Agar 15 g - -
Distilled water 1000 ml - -
1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131).
Solution occurs in 5 other media (first defined in Medium 215).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion
(Difco)
37 g 37 -
Distilled water 1000 ml - -

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 9
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Horse blood 40 -
Sheep blood 40 -
Glucose 10 55.506
Cysteine 1 8.254
Columbia agar base - -

Cultivation metadata from 9 strains BacDive

pH 6 - 8 1 strain
phyla 1 9 strains
oxygen aerobe 2 strains
oxygen microaerophile 2 strains
oxygen anaerobe 1 strain
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