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Main sol. 429c

Internal Solution-ID: S871

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Columbia agar base 1000.0 ml - -
Horse blood OR Sheep blood 4.0 % 40 -
Glucose 1.0 % 10 55.506
Cysteine 0.1 % 1 8.254
1 Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215.
429c COLUMBIA-GLUCOSE-CYSTEINE-AGAR