429c .
COLUMBIA-GLUCOSE-CYSTEINE-AGAR
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia agar base | 1000.0 | ml | ||
|
|
Horse blood | 4.0 | % | ||
|
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Glucose | 1.0 | % | ||
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Cysteine | 0.1 | % | ||
| 1 Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50°C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. | |||||
Main sol. 220 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 15 | g | ||
|
|
Soy peptone | 5 | g | ||
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NaCl | 5 | g | ||
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Agar | 15 | g | ||
|
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Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid CM 131). | |||||