Main sol. 466
Internal Solution-ID: S958
Equipment needed: Wheaton tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase soy broth | 30.00 | g | 30 | - | |
| Yeast extract | 3.00 | g | 3 | - | |
| NaCl | 13.00 | g | 13 | 222.45 | |
| KCl | 0.34 | g | 0.34 | 4.561 | |
| MgCl2 x 6 H2O | 4.00 | g | 4 | 19.675 | |
| MgSO4 x 7 H2O | 3.45 | g | 3.45 | 13.997 | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.2 | |||||
| 2 Incubate under microaerophilic conditions | |||||
Media with this solution
| 466 | TSBY SALT MEDIUM |