Main sol. 469
Internal Solution-ID: S961
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 5.0 | g | 5 | - | |
| Malt extract | 5.0 | g | 5 | - | |
| K2HPO4 | 5.7 | g | 5.7 | 32.726 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| NH4H2PO4 | 1.2 | g | 1.2 | 10.432 | |
|
Catalase
(Sigma C-10) |
60.0 | mg | 0.06 | - | |
| Distilled water | 980.0 | ml | - | - | |
| 1 Adjust pH to 7.0. Add catalase and MgSO4 to 10 ml of distilled water each, and sterilize separately by filtration. Cool down autoclaved main solution to 50°C, and aseptically add catalase and MgSO4 solution. | |||||
Media with this solution
| 469 | YM-CATALASE MEDIUM |