Main sol. 480a
Internal Solution-ID: S979
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 10.00 | g | 9.98 | 170.774 | |
NH4Cl | 0.33 | g | 0.329 | 6.157 | |
CaCl2 x 2 H2O | 0.33 | g | 0.329 | 2.24 | |
MgCl2 x 6 H2O | 0.33 | g | 0.329 | 1.62 | |
KCl | 0.33 | g | 0.329 | 4.418 | |
KH2PO4 | 0.33 | g | 0.329 | 2.42 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Yeast extract | 0.10 | g | 0.1 | - | |
Na-acetate | 5.00 | g | 4.99 | 60.829 | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Sulfur
(powder) |
10.00 | g | 9.98 | 311.195 | |
Na2CO3 | 1.00 | g | 0.998 | 9.416 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.499 | 2.078 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Adjust pH to 5.9, dispense medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials containing already the appropriate amount of sulfur and sterilize medium by heating in a boiling water bath for 2- 3 hours on each of 3 successive days. Add vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 6.8 - 7.0, if necessary. |
480a | DESULFURELLA MEDIUM (BRACKISH WATER) |