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Main sol. 484

Internal Solution-ID: S986

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 100 ml - -
Solution B 860 ml - -
Solution C 10 ml - -
Solution D 20 ml - -
Solution E 10 ml - -
1 Autoclave solutions A, B and C separately and mix when cool. After autoclaving, add thiosulfate, trace elements and vitamins from sterile stock solutions to complete the medium. Adjust pH of complete medium to 7.3 - 7.6.
484 THIOMICROSPIRA THYASIRAE MEDIUM