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DSMZ

DSMZ 484 . THIOMICROSPIRA THYASIRAE MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 484 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 100 ml - -
Solution B 860 ml - -
Solution C 10 ml - -
Solution D 20 ml - -
Solution E 10 ml - -
1 Autoclave solutions A, B and C separately and mix when cool. After autoclaving, add thiosulfate, trace elements and vitamins from sterile stock solutions to complete the medium. Adjust pH of complete medium to 7.3 - 7.6.

Solution A #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 7.9 g 79 556.499
KH2PO4 1.5 g 15 110.225
Distilled water 100.0 ml - -
1 Adjust to pH 7.6.

Solution B #

860
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.4 g 0.465 8.695
MgSO4 x 7 H2O 0.1 g 0.116 0.472
NaCl 25.0 g 29.07 497.429
Agar , for solid medium 15.0 g 17.442 51.859
Distilled water 860.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution 10 ml - -

Solution D #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 g 250 1007.31
Distilled water 20 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 10 ml - -

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 484).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Nicotinic acid 20 mg 0.02 0.162
Pyridoxine hydrochloride 20 mg 0.02 0.097
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Calcium pantothenate 20 mg 0.02 0.042
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 1 mg 1.0e-3 7.4e-4
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 333).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3 - 7.6
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 25 427.789
Agar 15 44.599
Na2HPO4 x 2 H2O 7.9 55.65
Na2S2O3 x 5 H2O 5 20.146
KH2PO4 1.5 11.023
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain