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Trace element solution

Internal Solution-ID: S612

This solution is used in 6 mediaand was first defined in medium 333.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.
333 THERMITHIOBACILLUS MEDIUM
389 ANNWOODIA MEDIUM
484 THIOMICROSPIRA THYASIRAE MEDIUM
486 THIOBACILLUS THIOPARUS (TK-m) MEDIUM
518 GUYPARKERIA MEDIUM
1734 THIOMICROSPIRA SP. MEDIUM