333
333 .
THERMITHIOBACILLUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 333 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.4 | g | 0.4 | 7.478 | |
| MgSO4 x 7 H2O | 0.8 | g | 0.8 | 3.246 | |
| Trace element solution | 10.0 | ml | - | - | |
| KH2PO4 | 4.0 | g | 4 | 29.393 | |
| K2HPO4 | 4.0 | g | 4 | 22.965 | |
| K2S4O6 | 3.0 | g | 3 | - | |
|
Bromocresol purple
(saturated aq. solution) |
2.0 | ml | - | - | |
|
Agar
, for solid medium
(BD Bacto) |
10.0 | g | 10 | 29.733 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except phosphates and tetrathionate. The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. Tetrathionate is sterilized by filtration and added to the autoclaved medium. Adjust pH of complete medium to 6.9. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately. | |||||
Solution occurs in 5
other media (first defined in Medium 333).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2-EDTA | 50.00 | g | 50 | 148.718 | |
| ZnSO4 x 7 H2O | 11.00 | g | 11 | 38.253 | |
| CaCl2 x 2 H2O | 7.34 | g | 7.34 | 49.927 | |
| MnCl2 x 4 H2O | 2.50 | g | 2.5 | 12.632 | |
| CoCl2 x 6 H2O | 0.50 | g | 0.5 | 3.851 | |
| (NH4)6Mo7O24 x 4 H2O | 0.50 | g | 0.5 | - | |
| FeSO4 x 7 H2O | 5.00 | g | 5 | 17.985 | |
| CuSO4 x 5 H2O | 0.20 | g | 0.2 | 0.801 | |
| NaOH | 11.00 | g | 11 | 275.021 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH. | |||||
Metadata
| Last modified: | 25.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.9 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 10 | 29.733 |
| KH2PO4 | 4 | 29.393 |
| K2HPO4 | 4 | 22.965 |
| K2S4O6 | 3 | - |
| MgSO4 x 7 H2O | 0.8 | 3.246 |
| Show more | ||
