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DSMZ

DSMZ 1734 . THIOMICROSPIRA SP. MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1734 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 17.50 g 17.5 299.452
NH4Cl 0.40 g 0.4 7.478
MgSO4 x 7 H2O 0.10 g 0.1 0.406
Trace element solution 10.00 ml - -
Na2S2O3 x 5 H2O 10.00 g 10 40.292
KH2PO4 0.14 g 0.14 1.029
NaHCO3 0.50 g 0.5 5.952
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, hydrogencarbonate and vitamins), adjust pH to 7.5 and autoclave. K2HPO4 is autoclaved separately. Filter sterilize the vitamins and NaHCO3 stock solutions. Adjust pH of the complete medium to 8.0 with sterile 0.4% (w/v) Na2CO3 solution, if necessary.
2 Note: Incubate in Erlenmeyer flasks with shaking.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 333).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 12.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 17.5 299.452
Na2S2O3 x 5 H2O 10 40.292
NaHCO3 0.5 5.952
Na2-EDTA 0.5 1.487
NH4Cl 0.4 7.478
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain