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DSMZ

DSMZ 486 . THIOBACILLUS THIOPARUS (TK-m) MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 486 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 2.0 g 2 14.697
K2HPO4 2.0 g 2 11.483
NH4Cl 0.4 g 0.4 7.478
Na2CO3 0.4 g 0.4 3.774
MgCl2 x 6 H2O 0.2 g 0.2 0.984
Vitamin solution 3.0 ml - -
Trace element solution 1.0 ml - -
Bromocresol purple
(saturated aq. solution) 		2.0 ml - -
Na2S2O3 x 5 H2O 5.0 g 5 20.146
Agar 15.0 g 15 44.599
Distilled water 1000.0 ml - -
1 Sterilize the phosphates separately in 1/10 of medium volume and mix with the other salts when cool. Add filter-sterilized vitamin solution after autoclaving to sterile medium. Adjust pH of complete medium to 7.1, if necessary.

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium 484).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine-HCl x 2 H2O 10 mg 0.01 0.027
Nicotinic acid 20 mg 0.02 0.162
Pyridoxine hydrochloride 20 mg 0.02 0.097
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Calcium pantothenate 20 mg 0.02 0.042
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 1 mg 1.0e-3 7.4e-4
Distilled water 1000 ml - -
1 Adjust pH to 7.0.

Trace element solution #

1000
Solution occurs in 5 other media (first defined in Medium 333).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 11.00 g 11 38.253
CaCl2 x 2 H2O 7.34 g 7.34 49.927
MnCl2 x 4 H2O 2.50 g 2.5 12.632
CoCl2 x 6 H2O 0.50 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.50 g 0.5 -
FeSO4 x 7 H2O 5.00 g 5 17.985
CuSO4 x 5 H2O 0.20 g 0.2 0.801
NaOH 11.00 g 11 275.021
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Metadata

Last modified: 28.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2S2O3 x 5 H2O 5 20.146
KH2PO4 2 14.697
K2HPO4 2 11.483
NH4Cl 0.4 7.478
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains