Main sol. 486
Internal Solution-ID: S993
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 2.0 | g | 2 | 14.697 | |
| K2HPO4 | 2.0 | g | 2 | 11.483 | |
| NH4Cl | 0.4 | g | 0.4 | 7.478 | |
| Na2CO3 | 0.4 | g | 0.4 | 3.774 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Vitamin solution | 3.0 | ml | - | - | |
| Trace element solution | 1.0 | ml | - | - | |
|
Bromocresol purple
(saturated aq. solution) |
2.0 | ml | - | - | |
| Na2S2O3 x 5 H2O | 5.0 | g | 5 | 20.146 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Sterilize the phosphates separately in 1/10 of medium volume and mix with the other salts when cool. Add filter-sterilized vitamin solution after autoclaving to sterile medium. Adjust pH of complete medium to 7.1, if necessary. | |||||
Media with this solution
| 486 | THIOBACILLUS THIOPARUS (TK-m) MEDIUM |