Name: GUYPARKERIA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 518 #

Equipment needed: Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂HPO₄ x 2 H₂O	7.9	g	7.9	55.65
KH₂PO₄	1.5	g	1.5	11.023
NaCl	50.0	g	50	855.578
MgSO₄ x 7 H₂O	0.1	g	0.1	0.406
NH₄Cl	0.4	g	0.4	7.478
Phenol red (saturated aq. solution)	12.5	ml	-	-
Trace element solution	10.0	ml	-	-
Na₂S₂O₃ x 5 H₂O	5.0	g	5	20.146
Agar , for solid medium optional	15.0	g	15	44.599
Distilled water	1000.0	ml	-	-
1 Dissolve ingredients except thiosulfate and adjust pH of medium to 7.3 - 7.5. Add thiosulfate to the medium after autoclaving from a sterile stock solution sterilized by filtration. For liquid medium also sterilize the phosphates separately in 1/10 of medium volume.

Trace element solution #

1000

Solution occurs in 5 other media (first defined in Medium 333).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂-EDTA	50.00	g	50	148.718
ZnSO₄ x 7 H₂O	11.00	g	11	38.253
CaCl₂ x 2 H₂O	7.34	g	7.34	49.927
MnCl₂ x 4 H₂O	2.50	g	2.5	12.632
CoCl₂ x 6 H₂O	0.50	g	0.5	3.851
(NH₄)₆Mo₇O₂₄ x 4 H₂O	0.50	g	0.5	-
FeSO₄ x 7 H₂O	5.00	g	5	17.985
CuSO₄ x 5 H₂O	0.20	g	0.2	0.801
NaOH	11.00	g	11	275.021
Distilled water	1000.00	ml	-	-
1 Dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. Adjust pH of final solution to 6.0 with NaOH.

Last modified:	22.02.22
Source:	DSMZ
Synonyms:	HALOTHIOBACILLUS HALOPHILUS MEDIUM
Taxonomic range:	Bacteria
Medium type:	Defined medium
Final pH:	7.3 - 7.5
Equipment needed:	Autoclave, Filter

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