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Solution A

Internal Solution-ID: S99

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.5 g 0.51 2.929
NH4Cl 1.0 g 1.02 19.077
Na2SO4 1.0 g 1.02 7.184
CaCl2 x 2 H2O 0.1 g 0.102 0.694
MgSO4 x 7 H2O 2.0 g 2.041 8.28
Na-DL-lactate 2.0 g 2.041 18.212
Yeast extract 1.0 g 1.02 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
Sea water
(filtered and aged)
980.0 ml - -
1 Alternatively to filtered aged sea water bottled water from Biomaris GmbH (Bremen, Germany) can be used.
63b DESULFOVIBRIO MEDIUM (SEAWATER)