Main sol. 487
Internal Solution-ID: S994
Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Betaine x H2O | 6.00 | g | 5.982 | 51.065 | |
| L-Alanine | 2.20 | g | 2.193 | 24.62 | |
| NaCl | 10.00 | g | 9.97 | 170.604 | |
| MgSO4 x 7 H2O | 0.50 | g | 0.499 | 2.023 | |
| NH4Cl | 1.00 | g | 0.997 | 18.639 | |
| CaCl2 x 2 H2O | 0.03 | g | 0.03 | 0.203 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.997 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 5.00 | g | 4.985 | 59.341 | |
| K2HPO4 | 0.36 | g | 0.359 | 2.061 | |
| KH2PO4 | 0.23 | g | 0.229 | 1.685 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.30 | g | 0.299 | 1.245 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, phosphates, vitamins and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add and dissolve bicarbonate, adjust pH to 7.5, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add phosphates, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.3 - 7.5, if necessary. | |||||
Media with this solution
| 487 | PEPTOCLOSTRIDIUM MEDIUM (BETAINE) |