Main sol. 588
Internal Solution-ID: S1263
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 87.800 | g | 87.8 | 1502.4 | |
| MgCl2 x 6 H2O | 20.300 | g | 20.3 | 99.851 | |
| CaCl2 x 2 H2O | 7.350 | g | 7.35 | 49.995 | |
| KCl | 3.700 | g | 3.7 | 49.631 | |
| Yeast extract | 5.000 | g | 5 | - | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.5 | 2.847 | |
| Resazurin | 0.100 | mg | 1.0e-4 | 4.4e-4 | |
| Glucose | 5.000 | g | 5 | 27.753 | |
| PIPES | 12.096 | g | 12.096 | 40.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 pH 6.5 | |||||
| 2 Prepare salts (in 850 ml distilled water, do not adjust the pH), glucose (50 ml of a 10% solution), and PIPES (100 ml of 400 mM solution, 12.096 g/100 ml), separately under nitrogen using normal anaerobic techniques. Add glucose and PIPES using anaerobic techniques. | |||||
Media with this solution
| 588 | HALOBACTEROIDES HALOBIUS MEDIUM |