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Main sol. 670

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Internal Solution-ID: S1413

Equipment needed: Autoclave Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 2.00 g 2 15.136
MgSO4 x 7 H2O 0.25 g 0.25 1.014
K2HPO4 0.10 g 0.1 0.574
KCl 0.10 g 0.1 1.341
FeSO4 x 7 H2O
(40% w/v in 0.2 N H2SO4) 		50.00 ml 20 71.939
Distilled water 950.00 ml - -
1 Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.
2 Note: Incubate statically without shaking.
670 ACIDIFERROBACTER (MS) MEDIUM