Main sol. 800
Internal Solution-ID: S1630
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Wilkins Chalgrene Anaerobe Broth
(Oxoid CM 643) |
10.0 | ml | - | - | |
| 1 Use Wilkins Chalgrene Anaerobe Broth (Oxoid CM 643) and prepare it anaerobically under 80% N2 + 20% CO2 gas. After autoclaving and cooling to room temperature add per 10 ml the following sterile, anaerobic solutions: | |||||
|
5-Aminovaleric acid
(w/v) NaHCO3 (flushed with N2 + CO2) |
0.5 | ml | - | - | |
|
Fildes Enrichment
(BBL 20810) (under N2) |
1.0 | ml | - | - | |
|
3-Hydroxybenzoic acid
(w/v) cysteine-HCl x H2O (under N2) |
0.1 | ml | - | - | |
|
3-Hydroxybenzoic acid
(w/v) Na2S x 9 H2O (under N2) |
0.1 | ml | - | - | |
| 2 The pH of the completed medium should be 6.8 - 7.0. For growth an agar plates, Columbia sheep blood agar plates (BBL) may be used and incubated under an anaerobic atmosphere containig 5 - 10% CO2. | |||||
Media with this solution
| 800 | ANAEROBIOSPIRILLUM THOMASII MEDIUM |