Liquid medium (in 5ml tube)
Internal Solution-ID: S1718
Equipment needed: Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase soy broth
(BBL 11774) |
5.00 | ml | - | - | |
| 1 Prepare Trypticase Soy Broth (BBL 11774) under 80% N2 + 20% CO2 gas mixture in Hungate tubes (5 ml per tube). | |||||
| 2 After autoclaving and cooling add (per tube): | |||||
|
NaHCO3
(10% w/v; sterile) |
0.30 | ml | 0.03 | 0.357 | |
|
Bovine serum
(sterile; heat inactivated at 56°C for 45 min) |
0.35 | ml | - | - | |
|
Glucose
(30% w/v; sterile) |
0.05 | ml | 0.015 | 0.083 | |
| Air | 0.50 | ml | - | - | |
| 3 Incubate inoculated tubes in slanted position. | |||||
Media with this solution
| 840 | SERPULINA MURDOCHII MEDIUM |