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Main sol. 1275

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Internal Solution-ID: S2545

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 0.50 ml - -
Solution C 0.50 ml - -
Solution D 0.25 ml - -
Solution E 0.40 ml - -
1 Solutions are prepared anaerobically under N2:CO2. Solutions A, B, C and D are autoclaved. After cooling, the solutions are combined under N2 gas. To 10 ml solution A, add 0.5 ml solution B, 0.5 ml solution C, 0.25 ml solution D and 0.4 ml solution E.
2 pH should be 7.3 without further adjustment. Add new toluene solution to the cultures about every 7 days.
1275 GEORGFUCHSIA MEDIUM