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DSMZ

DSMZ 1275 . GEORGFUCHSIA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1275 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 0.50 ml - -
Solution C 0.50 ml - -
Solution D 0.25 ml - -
Solution E 0.40 ml - -
1 Solutions are prepared anaerobically under N2:CO2. Solutions A, B, C and D are autoclaved. After cooling, the solutions are combined under N2 gas. To 10 ml solution A, add 0.5 ml solution B, 0.5 ml solution C, 0.25 ml solution D and 0.4 ml solution E.
2 pH should be 7.3 without further adjustment. Add new toluene solution to the cultures about every 7 days.

Solution A #

905
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.65 g 0.718 5.278
Na2HPO4 0.20 g 0.221 1.557
Resazurin 0.50 mg 5.5e-4 0.002
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 4.00 ml - -
Distilled water 900.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 0.11 g 2.2 19.823
MgCl2 0.10 g 2 21.006
Distilled water 50.00 ml - -
Vitamin solution 1.00 ml - -
1 Add 1 ml vitamin solution (see below) after autoclaving and cooling.

Solution C #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4HCO3 0.44 g 8.8 -
NaHCO3 3.73 g 74.6 888.021
L-Cysteine HCl x H2O 0.03 g 0.6 3.416
Distilled water 50.00 ml - -

Solution D #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaNO3 3.4 g 34 400.024
Distilled water 100.0 ml - -

Solution E #

100
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Toluene 58 µl - -
Distilled water 100 ml - -
1 Sterile distilled water gassed with dinitrogen, 100.00 ml. Add 58 µl toluene.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 178 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
D-Calcium pantothenate 50 mg 0.05 -
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.391 40.365
NaNO3 0.773 9.091
KH2PO4 0.653 4.798
NH4HCO3 0.4 -
Na2HPO4 0.201 1.415
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Cultivation metadata from 0 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain