Main sol. 158
Internal Solution-ID: S273
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 1.320 | g | 1.32 | 9.989 | |
KH2PO4 | 0.372 | g | 0.372 | 2.734 | |
MgSO4 x 7 H2O | 0.247 | g | 0.247 | 1.002 | |
CaCl2 x 2 H2O | 0.074 | g | 0.074 | 0.503 | |
Trace element solution | 10.000 | ml | - | - | |
Yeast extract
(Oxoid) |
2.000 | g | 2 | - | |
Glucose | 20.000 | g | 20 | 111.012 | |
Distilled water
(freshly distilled) |
1000.000 | ml | - | - | |
1 Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium. |
158 | THERMOPLASMA ACIDOPHILUM MEDIUM |