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Main sol. 158

Internal Solution-ID: S273

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.320 g 1.32 9.989
KH2PO4 0.372 g 0.372 2.734
MgSO4 x 7 H2O 0.247 g 0.247 1.002
CaCl2 x 2 H2O 0.074 g 0.074 0.503
Trace element solution 10.000 ml - -
Yeast extract
(Oxoid)
2.000 g 2 -
Glucose 20.000 g 20 111.012
Distilled water
(freshly distilled)
1000.000 ml - -
1 Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium.
158 THERMOPLASMA ACIDOPHILUM MEDIUM