Vitamin mix (pfennig & Lippert,1966)
Internal Solution-ID: S3031
This solution is used in 2 media and was first defined in medium 1479.
Internal Solution-ID: S3031
This solution is used in 2 media and was first defined in medium 1479.
1 1: acidic solution (HCl, pH3; 100ml) | |||||
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Thiamin | 5.0 | mg | 0.013 | 0.047 | |
Calcium pantothenate | 5.0 | mg | 0.013 | 0.026 | |
2 2: basic solution (100ml 0.1N NaOH) | |||||
Biotin | 2.0 | mg | 0.005 | 0.02 | |
PABA
(p-aminobenzoic acid) |
5.0 | mg | 0.013 | 0.091 | |
Nicotinic acid | 5.0 | mg | 0.013 | 0.102 | |
Pyridoxine | 10.0 | mg | 0.025 | 0.148 | |
3 3: Neutral solution in 100 ml demi water | |||||
Folic acid | 2.0 | mg | 0.005 | 0.011 | |
Riboflavin | 5.0 | mg | 0.013 | 0.033 | |
4 4: neutral solution in 100 ml demi water | |||||
B12 | 0.5 | mg | 0.001 | 9.2e-4 | |
5 Sterilize by filtration. | |||||
6 Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L. |