1491
1491 .
CYCLONATRONUM PROTEIVORANS MEDIUM (OMEGA)
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1491 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 5 | g | 5 | 47.175 | |
| NaCl | 10 | g | 10 | 171.116 | |
| K2HPO4 | 1 | g | 1 | 5.741 | |
| Trace element solution | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Prepare the liquid medium. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: | |||||
| Yeast extract | 20 | mg | 0.02 | - | |
| Peptone | 2 | g | 2 | - | |
| Vitamin mix (pfennig & Lippert,1966) | 1 | ml | - | - | |
| 2 pH 10.0 - 10.1 | |||||
Solution occurs in 3
other media (first defined in Medium 1478).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| EDTA | 5.00 | g | 5 | 17.109 | |
| FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 | |
| ZnSO4 | 0.10 | g | 0.1 | 0.62 | |
| MnCl2 | 0.03 | g | 0.03 | 0.238 | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 5 H2O | 0.01 | g | 0.01 | 0.045 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.02 | g | 0.02 | 0.083 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.0-4.0 with HCl. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 Note: autoclave in closed bottles | |||||
Solution occurs in 1
other media (first defined in Medium 1479).
Equipment needed: Filter
| 1 1: acidic solution (HCl, pH3; 100ml) | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Thiamin | 5.0 | mg | 0.013 | 0.047 | |
| Calcium pantothenate | 5.0 | mg | 0.013 | 0.026 | |
| 2 2: basic solution (100ml 0.1N NaOH) | |||||
| Biotin | 2.0 | mg | 0.005 | 0.02 | |
|
PABA
(p-aminobenzoic acid) |
5.0 | mg | 0.013 | 0.091 | |
| Nicotinic acid | 5.0 | mg | 0.013 | 0.102 | |
| Pyridoxine | 10.0 | mg | 0.025 | 0.148 | |
| 3 3: Neutral solution in 100 ml demi water | |||||
| Folic acid | 2.0 | mg | 0.005 | 0.011 | |
| Riboflavin | 5.0 | mg | 0.013 | 0.033 | |
| 4 4: neutral solution in 100 ml demi water | |||||
| B12 | 0.5 | mg | 0.001 | 9.2e-4 | |
| 5 Sterilize by filtration. | |||||
| 6 Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L. | |||||
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Medium type: | Complex medium |
| Final pH: | 10.0 - 10.1 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |