Trace element solution
Internal Solution-ID: S3027
This solution is used in 4 media and was first defined in medium 1478.
Internal Solution-ID: S3027
This solution is used in 4 media and was first defined in medium 1478.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
EDTA | 5.00 | g | 5 | 17.109 | |
FeSO4 x 7 H2O | 2.00 | g | 2 | 7.194 | |
ZnSO4 | 0.10 | g | 0.1 | 0.62 | |
MnCl2 | 0.03 | g | 0.03 | 0.238 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 5 H2O | 0.01 | g | 0.01 | 0.045 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.02 | g | 0.02 | 0.083 | |
Distilled water | 1000.00 | ml | - | - | |
1 Adjust pH to 3.0-4.0 with HCl. | |||||
2 Autoclave at 121 °C for 15 min. | |||||
3 Note: autoclave in closed bottles |