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Main sol. J414

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Internal Solution-ID: S4147

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1000 7348.29
K2HPO4 1.00 g 1000 5741.32
NH4Cl 1.00 g 1000 18695.1
CaCl2 x 2 H2O 0.10 g 100 680.203
MgCl2 x 6 H2O 0.20 g 200 983.753
Na2S2O3 x 5 H2O 1.55 g 1550 6245.32
Trace metal solution 1.00 ml - -
NaHCO3 2.00 g 2000 23807.5
1 Dissolve KH2PO4, K2HPO4, NH4Cl and Na2S2O3 x 5H2O in 500 ml distilled water. Separately dissolve CaCl2 x 2H2O, MgCl2 x 6H2O and Trace metal solution in 460 ml distilled water. Autoclave both solutions, mix them after cooling, and add 40 ml filter-sterilized 5% NaHCO3 solution to the medium. Adjust pH to 7.0 with HCl, if necessary.
J414 MODIFIED SOB MEDIUM