MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 414 . MODIFIED SOB MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J414 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1000 7348.29
K2HPO4 1.00 g 1000 5741.32
NH4Cl 1.00 g 1000 18695.1
CaCl2 x 2 H2O 0.10 g 100 680.203
MgCl2 x 6 H2O 0.20 g 200 983.753
Na2S2O3 x 5 H2O 1.55 g 1550 6245.32
Trace metal solution 1.00 ml - -
NaHCO3 2.00 g 2000 23807.5
1 Dissolve KH2PO4, K2HPO4, NH4Cl and Na2S2O3 x 5H2O in 500 ml distilled water. Separately dissolve CaCl2 x 2H2O, MgCl2 x 6H2O and Trace metal solution in 460 ml distilled water. Autoclave both solutions, mix them after cooling, and add 40 ml filter-sterilized 5% NaHCO3 solution to the medium. Adjust pH to 7.0 with HCl, if necessary.

Trace metal solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.0 g 50 -
ZnSO4 x 7 H2O 2.2 g 2.2 7.651
CaCl2 x 2 H2O 7.3 g 7.3 49.655
MgCl2 x 6 H2O 2.5 g 2.5 12.297
CoCl2 x 6 H2O 0.5 g 0.5 3.851
(NH4)6Mo7O24 x 4 H2O 0.5 g 0.5 -
FeSO4 x 7 H2O 5.0 g 5 17.985
CuSO4 x 5 H2O 0.2 g 0.2 0.801
NaOH 11.0 g 11 275.021
Distilled water 1000.0 ml - -
1 Adjust pH to 6.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2000 23807.5
Na2S2O3 x 5 H2O 1550 6245.32
NH4Cl 1000 18695.1
KH2PO4 1000 7348.29
K2HPO4 1000 5741.32
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain