MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. J438

Download this solution as

CSV JSON PDF

Internal Solution-ID: S4184

Equipment needed: Filter Autoclave Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.00 g 500 8555.78
MgCl2 x 6 H2O 0.10 g 50 245.938
CaCl2 x 2 H2O 0.05 g 25 170.051
NH4Cl 0.25 g 125 2336.89
KH2PO4 0.10 g 50 367.415
KCl 0.50 g 250 3353.45
Yeast extract 0.50 g 250 -
CAPSO 11.86 g 5930 -
Na2SO4 4.00 g 2000 14080.5
Trace element solution
(see Medium No. 294) 		1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium lactate 2.24 g 1120 9994.65
Resazurin 1.00 mg 0.5 2.182
Sodium dithionite 0.10 g 50 287.175
1 Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution.
J438 RT2 MEDIUM