Main sol. J561
Internal Solution-ID: S4383
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.49 | 3.599 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.147 | 0.999 | |
| MgSO4 x 7 H2O | 2.00 | g | 1.959 | 7.947 | |
| NH4Cl | 0.34 | g | 0.333 | 6.226 | |
| NaCl | 20.00 | g | 19.589 | 335.192 | |
| Yeast extract | 0.40 | g | 0.392 | - | |
| Sodium pyruvate | 3.00 | g | 2.938 | 26.702 | |
| Micronutrient solution SL7 | 1.00 | ml | - | - | |
|
Ferric citrate
(0.1%, w/v) |
5.00 | ml | - | - | |
|
NaHCO3
(10%, w/v) |
15.00 | ml | - | - | |
| Na2S x 9 H2O | 1.00 | mM | 0.24 | 1 | |
| Na2S2O3 x 5 H2O | 6.00 | mM | 1.489 | 6 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Autoclave except NaHCO3, Na2S x 9H2O and Na2S2O3 x 5H2O. After cooling, aseptically add filter-sterilized NaHCO3, Na2S x 9H2O and Na2S2O3 x 5H2O solutions to the medium. | |||||
Media with this solution
| J561 | THIORHODOCOCCUS BHEEMLICUM MEDIUM |