Main sol. J1253
Internal Solution-ID: S5390
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 166.667 | 1224.72 | |
| KCl | 1.00 | g | 166.667 | 2235.64 | |
| NH4Cl | 1.00 | g | 166.667 | 3115.85 | |
| MgSO4 x 7 H2O | 0.24 | g | 40 | 162.288 | |
| CaSO4 x 2 H2O | 0.17 | g | 28.333 | 164.564 | |
| SL-6 trace element solution | 1.00 | ml | - | - | |
| NaCl | 140.00 | g | 23333.3 | 399270 | |
| Na glutamate | 1.00 | g | 166.667 | 872.189 | |
|
Yeast extract
(BD-Difco) |
5.00 | g | 833.333 | - | |
|
Casamino acids
(BD-Difco) |
5.00 | g | 833.333 | - | |
| Agar | 20.00 | g | 3333.33 | 9910.9 | |
| 1 Add components to distilled water and bring volume to 1 L. Adjust pH to 6.5 and autoclave. Aseptically add the following solution (autoclaved) to the medium: | |||||
|
Na2CO3
(w/v, 10%) |
5.00 | ml | - | - | |
| 2 Check final pH of the medium to be 7.5-8.0. | |||||
Media with this solution
| J1253 | SALICIBIBACTER MEDIUM |