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JCM 1253 . SALICIBIBACTER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1253 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 166.667 1224.72
KCl 1.00 g 166.667 2235.64
NH4Cl 1.00 g 166.667 3115.85
MgSO4 x 7 H2O 0.24 g 40 162.288
CaSO4 x 2 H2O 0.17 g 28.333 164.564
SL-6 trace element solution 1.00 ml - -
NaCl 140.00 g 23333.3 399270
Na glutamate 1.00 g 166.667 872.189
Yeast extract
(BD-Difco) 		5.00 g 833.333 -
Casamino acids
(BD-Difco) 		5.00 g 833.333 -
Agar 20.00 g 3333.33 9910.9
1 Add components to distilled water and bring volume to 1 L. Adjust pH to 6.5 and autoclave. Aseptically add the following solution (autoclaved) to the medium:
Na2CO3
(w/v, 10%) 		5.00 ml - -
2 Check final pH of the medium to be 7.5-8.0.

SL-6 trace element solution #

1000
Solution occurs in 10 other media (first defined in Medium J167).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 23333.3 399270
Agar 3333.33 9910.9
Yeast extract 833.333 -
Casamino acids 833.333 -
NH4Cl 166.667 3115.85
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain